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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts just isn't yet readily available.
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Blocking peptides are peptides that bind specially towards the concentrate on antibody and block antibody binding. These peptide typically incorporates the epitope acknowledged via the antibody.
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This guideline outlines even further doable optimization of cellular and immunoassay parameters to make sure the best possible success are...
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This tutorial outlines more possible optimization of cellular and immunoassay parameters to be certain the best possible final results are...
Antibodies certain to the blocking peptide not bind on the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins show distinct expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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